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TESTS
QubeMDx+™ Avian Sexing Examination Kit

determining avian sex based on external features can be challenging.

Accurate gender identification in animals is crucial for veterinary, medical, and ecological research. However, determining sex based on external features can be challenging, as over 50% of bird species are monomorphic, meaning males and females appear identical.

Traditional methods, such as observing sex-specific behaviors, laparoscopy, laparotomy, or cloacal examination, are often time-consuming, costly, and, in some cases, invasive or risky.

QubeMDx+
Avian Sexing Examination Kit

REGISTRATION STATUS

Research Use Only

To overcome the limitations of traditional sexing methods, molecular sexing techniques using non-invasive samples like feathers have been developed. The difference in Chromo-Helicase DNA-binding (CHD) gene length between the Z and W chromosomes distinguishes males (ZZ) from females (ZW). This method enables reliable sex identification from unknown samples.

The most suitable primer set can be selected based on the species to be sexed, or multiple sets can be tested for the best match. Each primer set works for a range of species, as listed below [1]. Note that this list is a guide, not exhaustive. Please consult the relevant literature for your avian species or contact us for further assistance.

Overcoming the Limitations of Traditional Sexing Methods

Ready to Use

Rapid Result

Ambient Storage

Diagnostic Procedure

QubeMDx+™ Avian Sexing Examination Test

Catalog No.
PCRCR4101  (primer set: P2/P8/P0)
PCRCR4102 (primer set: 2550F/2718R)
PCRCR4103 (primer set: CHD1F/CHD1R)

Technology
PCR 

Hands-on Time
< 2 min

Turnaround Time
Approximately 1 hour

Kit Storage
5 – 25 °C / 41 – 77 °F

Quantity
48 tests/box

Targets
CHD-W / CHD-Z gene

Sample Types
Feather / Blood

Reference
[1] Optimal primer sets for avian species are referenced from: Çakmak, Emel, ÇiğdemAkınPekşen, and C. Can Bilgin, “Comparison of three different primer sets for sexing birds,” Journal of Veterinary Diagnostic Investigation 29.1 (2017): 59-63)